Injectable multifunctional microgel encapsulating outgrowth endothelial cells and growth factors for enhanced neovascularization.
Identifieur interne : 001A52 ( Main/Exploration ); précédent : 001A51; suivant : 001A53Injectable multifunctional microgel encapsulating outgrowth endothelial cells and growth factors for enhanced neovascularization.
Auteurs : Pyung-Hwan Kim [Corée du Sud] ; Hyun-Gu Yim [Corée du Sud] ; Young-Jin Choi [Corée du Sud] ; Byung-Jae Kang [Corée du Sud] ; Joohyun Kim [Corée du Sud] ; Sang-Mo Kwon [Corée du Sud] ; Byung-Soo Kim [Corée du Sud] ; Nathaniel S. Hwang [Corée du Sud] ; Je-Yoel Cho [Corée du Sud]Source :
- Journal of controlled release : official journal of the Controlled Release Society [ 1873-4995 ] ; 2014.
Descripteurs français
- KwdFr :
- Acide glucuronique (composition chimique), Acides hexuroniques (composition chimique), Alginates (composition chimique), Animaux (MeSH), Aorte (physiologie), Cellules HEK293 (MeSH), Cellules cultivées (MeSH), Cellules endothéliales (MeSH), Cellules endothéliales de la veine ombilicale humaine (MeSH), Débit sanguin régional (effets des médicaments et des substances chimiques), Facteur de croissance des hépatocytes (administration et posologie), Facteur de croissance endothéliale vasculaire de type A (administration et posologie), Femelle (MeSH), Fluorescéine-5-isothiocyanate (administration et posologie), Fluorescéine-5-isothiocyanate (composition chimique), Gels (MeSH), Humains (MeSH), Injections (MeSH), Ischémie (traitement médicamenteux), Membres (vascularisation), Néovascularisation physiologique (effets des médicaments et des substances chimiques), Oligopeptides (composition chimique), Rat Sprague-Dawley (MeSH), Souris de lignée BALB C (MeSH), Sérumalbumine bovine (administration et posologie), Sérumalbumine bovine (composition chimique), Thérapie cellulaire et tissulaire (méthodes).
- MESH :
- administration et posologie : Facteur de croissance des hépatocytes, Facteur de croissance endothéliale vasculaire de type A, Fluorescéine-5-isothiocyanate, Sérumalbumine bovine.
- composition chimique : Acide glucuronique, Acides hexuroniques, Alginates, Fluorescéine-5-isothiocyanate, Oligopeptides, Sérumalbumine bovine.
- effets des médicaments et des substances chimiques : Débit sanguin régional, Néovascularisation physiologique.
- méthodes : Thérapie cellulaire et tissulaire.
- physiologie : Aorte.
- traitement médicamenteux : Ischémie.
- vascularisation : Membres.
- Animaux, Cellules HEK293, Cellules cultivées, Cellules endothéliales, Cellules endothéliales de la veine ombilicale humaine, Femelle, Gels, Humains, Injections, Rat Sprague-Dawley, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Alginates (chemistry), Animals (MeSH), Aorta (physiology), Cell- and Tissue-Based Therapy (methods), Cells, Cultured (MeSH), Endothelial Cells (MeSH), Extremities (blood supply), Female (MeSH), Fluorescein-5-isothiocyanate (administration & dosage), Fluorescein-5-isothiocyanate (chemistry), Gels (MeSH), Glucuronic Acid (chemistry), HEK293 Cells (MeSH), Hepatocyte Growth Factor (administration & dosage), Hexuronic Acids (chemistry), Human Umbilical Vein Endothelial Cells (MeSH), Humans (MeSH), Injections (MeSH), Ischemia (drug therapy), Mice, Inbred BALB C (MeSH), Neovascularization, Physiologic (drug effects), Oligopeptides (chemistry), Rats, Sprague-Dawley (MeSH), Regional Blood Flow (drug effects), Serum Albumin, Bovine (administration & dosage), Serum Albumin, Bovine (chemistry), Vascular Endothelial Growth Factor A (administration & dosage).
- MESH :
- chemical , administration & dosage : Fluorescein-5-isothiocyanate, Hepatocyte Growth Factor, Serum Albumin, Bovine, Vascular Endothelial Growth Factor A.
- chemical , chemistry : Alginates, Fluorescein-5-isothiocyanate, Glucuronic Acid, Hexuronic Acids, Oligopeptides, Serum Albumin, Bovine.
- blood supply : Extremities.
- drug effects : Neovascularization, Physiologic, Regional Blood Flow.
- drug therapy : Ischemia.
- methods : Cell- and Tissue-Based Therapy.
- physiology : Aorta.
- Animals, Cells, Cultured, Endothelial Cells, Female, Gels, HEK293 Cells, Human Umbilical Vein Endothelial Cells, Humans, Injections, Mice, Inbred BALB C, Rats, Sprague-Dawley.
Abstract
Recent cell-based therapy approaches have employed both nanotechnologies and other biomedical technologies to enhance their therapeutic potential. A combined strategy using therapeutic stem/progenitor cells and angiogenic proteins is attractive for the treatment of vascular disease. In this study, we developed an injectable multifunctional micro-sized gel system (microgel), composed of arginine-glycine-aspartic acid (RGD)-conjugated alginate, for the delivery of both cells and growth factors in vivo. The microgels encapsulated with outgrowth endothelial cells (OECs) and growth factors (vascular endothelial growth factor, VEGF, and hepatocyte growth factor, HGF) were formed via electrospraying. Cells encapsulated within the microgel exhibited a time-dependent proliferation with enhanced cell viability, and the size-controlled microgels resulted in sustained release of growth factors for enhanced new vessel formation by tube formation and rat aorta sprouting in vitro. Increased angiogenesis was also estimated in mice treated with RGD-microgel containing OECs and growth factors. Furthermore, injection of the multifunctional microgel into a hindlimb ischemia model improved blood flow perfusion and increased the capillary density by histological analysis. Compared with hydrogel system, injectable microgel system was shown to be superior with no toxicity. Overall, our injectable multifunctional microgel system can be attributed to deliver potential therapeutic agents/cells for the treatment of vascular diseases.
DOI: 10.1016/j.jconrel.2014.05.010
PubMed: 24852096
Affiliations:
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Soo" sort="Kim, Byung Soo" uniqKey="Kim B" first="Byung-Soo" last="Kim">Byung-Soo Kim</name><affiliation wicri:level="4"><nlm:affiliation>School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Hwang, Nathaniel S" sort="Hwang, Nathaniel S" uniqKey="Hwang N" first="Nathaniel S" last="Hwang">Nathaniel S. Hwang</name><affiliation wicri:level="4"><nlm:affiliation>School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Cho, Je Yoel" sort="Cho, Je Yoel" uniqKey="Cho J" first="Je-Yoel" last="Cho">Je-Yoel Cho</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea. 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Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Yim, Hyun Gu" sort="Yim, Hyun Gu" uniqKey="Yim H" first="Hyun-Gu" last="Yim">Hyun-Gu Yim</name><affiliation wicri:level="4"><nlm:affiliation>School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Choi, Young Jin" sort="Choi, Young Jin" uniqKey="Choi Y" first="Young-Jin" last="Choi">Young-Jin Choi</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Kang, Byung Jae" sort="Kang, Byung Jae" uniqKey="Kang B" first="Byung-Jae" last="Kang">Byung-Jae Kang</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Kim, Joohyun" sort="Kim, Joohyun" uniqKey="Kim J" first="Joohyun" last="Kim">Joohyun Kim</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Kwon, Sang Mo" sort="Kwon, Sang Mo" uniqKey="Kwon S" first="Sang-Mo" last="Kwon">Sang-Mo Kwon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Physiology, School of Medicine, Pusan national University, Yangsan, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Physiology, School of Medicine, Pusan national University, Yangsan</wicri:regionArea><wicri:noRegion>Yangsan</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Byung Soo" sort="Kim, Byung Soo" uniqKey="Kim B" first="Byung-Soo" last="Kim">Byung-Soo Kim</name><affiliation wicri:level="4"><nlm:affiliation>School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Hwang, Nathaniel S" sort="Hwang, Nathaniel S" uniqKey="Hwang N" first="Nathaniel S" last="Hwang">Nathaniel S. Hwang</name><affiliation wicri:level="4"><nlm:affiliation>School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Chemical and Biological Engineering, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Cho, Je Yoel" sort="Cho, Je Yoel" uniqKey="Cho J" first="Je-Yoel" last="Cho">Je-Yoel Cho</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea. Electronic address: jeycho@snu.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author></analytic><series><title level="j">Journal of controlled release : official journal of the Controlled Release Society</title><idno type="eISSN">1873-4995</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alginates (chemistry)</term><term>Animals (MeSH)</term><term>Aorta (physiology)</term><term>Cell- and Tissue-Based Therapy (methods)</term><term>Cells, Cultured (MeSH)</term><term>Endothelial Cells (MeSH)</term><term>Extremities (blood supply)</term><term>Female (MeSH)</term><term>Fluorescein-5-isothiocyanate (administration & dosage)</term><term>Fluorescein-5-isothiocyanate (chemistry)</term><term>Gels (MeSH)</term><term>Glucuronic Acid (chemistry)</term><term>HEK293 Cells (MeSH)</term><term>Hepatocyte Growth Factor (administration & dosage)</term><term>Hexuronic Acids (chemistry)</term><term>Human Umbilical Vein Endothelial Cells (MeSH)</term><term>Humans (MeSH)</term><term>Injections (MeSH)</term><term>Ischemia (drug therapy)</term><term>Mice, Inbred BALB C (MeSH)</term><term>Neovascularization, Physiologic (drug effects)</term><term>Oligopeptides (chemistry)</term><term>Rats, Sprague-Dawley (MeSH)</term><term>Regional Blood Flow (drug effects)</term><term>Serum Albumin, Bovine (administration & dosage)</term><term>Serum Albumin, Bovine (chemistry)</term><term>Vascular Endothelial Growth Factor A (administration & dosage)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide glucuronique (composition chimique)</term><term>Acides hexuroniques (composition chimique)</term><term>Alginates (composition chimique)</term><term>Animaux (MeSH)</term><term>Aorte (physiologie)</term><term>Cellules HEK293 (MeSH)</term><term>Cellules cultivées (MeSH)</term><term>Cellules endothéliales (MeSH)</term><term>Cellules endothéliales de la veine ombilicale humaine (MeSH)</term><term>Débit sanguin régional (effets des médicaments et des substances chimiques)</term><term>Facteur de croissance des hépatocytes (administration et posologie)</term><term>Facteur de croissance endothéliale vasculaire de type A (administration et posologie)</term><term>Femelle (MeSH)</term><term>Fluorescéine-5-isothiocyanate (administration et posologie)</term><term>Fluorescéine-5-isothiocyanate (composition chimique)</term><term>Gels (MeSH)</term><term>Humains (MeSH)</term><term>Injections (MeSH)</term><term>Ischémie (traitement médicamenteux)</term><term>Membres (vascularisation)</term><term>Néovascularisation physiologique (effets des médicaments et des substances chimiques)</term><term>Oligopeptides (composition chimique)</term><term>Rat Sprague-Dawley (MeSH)</term><term>Souris de lignée BALB C (MeSH)</term><term>Sérumalbumine bovine (administration et posologie)</term><term>Sérumalbumine bovine (composition chimique)</term><term>Thérapie cellulaire et tissulaire (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Fluorescein-5-isothiocyanate</term><term>Hepatocyte Growth Factor</term><term>Serum Albumin, Bovine</term><term>Vascular Endothelial Growth Factor A</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alginates</term><term>Fluorescein-5-isothiocyanate</term><term>Glucuronic Acid</term><term>Hexuronic Acids</term><term>Oligopeptides</term><term>Serum Albumin, Bovine</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Facteur de croissance des hépatocytes</term><term>Facteur de croissance endothéliale vasculaire de type A</term><term>Fluorescéine-5-isothiocyanate</term><term>Sérumalbumine bovine</term></keywords><keywords scheme="MESH" qualifier="blood supply" xml:lang="en"><term>Extremities</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Acide glucuronique</term><term>Acides hexuroniques</term><term>Alginates</term><term>Fluorescéine-5-isothiocyanate</term><term>Oligopeptides</term><term>Sérumalbumine bovine</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Neovascularization, Physiologic</term><term>Regional Blood Flow</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Ischemia</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Débit sanguin régional</term><term>Néovascularisation physiologique</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cell- and Tissue-Based Therapy</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Thérapie cellulaire et tissulaire</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Aorte</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Aorta</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Ischémie</term></keywords><keywords scheme="MESH" qualifier="vascularisation" xml:lang="fr"><term>Membres</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Endothelial Cells</term><term>Female</term><term>Gels</term><term>HEK293 Cells</term><term>Human Umbilical Vein Endothelial Cells</term><term>Humans</term><term>Injections</term><term>Mice, Inbred BALB C</term><term>Rats, Sprague-Dawley</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HEK293</term><term>Cellules cultivées</term><term>Cellules endothéliales</term><term>Cellules endothéliales de la veine ombilicale humaine</term><term>Femelle</term><term>Gels</term><term>Humains</term><term>Injections</term><term>Rat Sprague-Dawley</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recent cell-based therapy approaches have employed both nanotechnologies and other biomedical technologies to enhance their therapeutic potential. A combined strategy using therapeutic stem/progenitor cells and angiogenic proteins is attractive for the treatment of vascular disease. In this study, we developed an injectable multifunctional micro-sized gel system (microgel), composed of arginine-glycine-aspartic acid (RGD)-conjugated alginate, for the delivery of both cells and growth factors in vivo. The microgels encapsulated with outgrowth endothelial cells (OECs) and growth factors (vascular endothelial growth factor, VEGF, and hepatocyte growth factor, HGF) were formed via electrospraying. Cells encapsulated within the microgel exhibited a time-dependent proliferation with enhanced cell viability, and the size-controlled microgels resulted in sustained release of growth factors for enhanced new vessel formation by tube formation and rat aorta sprouting in vitro. Increased angiogenesis was also estimated in mice treated with RGD-microgel containing OECs and growth factors. Furthermore, injection of the multifunctional microgel into a hindlimb ischemia model improved blood flow perfusion and increased the capillary density by histological analysis. Compared with hydrogel system, injectable microgel system was shown to be superior with no toxicity. Overall, our injectable multifunctional microgel system can be attributed to deliver potential therapeutic agents/cells for the treatment of vascular diseases. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24852096</PMID><DateCompleted><Year>2015</Year><Month>02</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4995</ISSN><JournalIssue CitedMedium="Internet"><Volume>187</Volume><PubDate><Year>2014</Year><Month>Aug</Month><Day>10</Day></PubDate></JournalIssue><Title>Journal of controlled release : official journal of the Controlled Release Society</Title><ISOAbbreviation>J Control Release</ISOAbbreviation></Journal><ArticleTitle>Injectable multifunctional microgel encapsulating outgrowth endothelial cells and growth factors for enhanced neovascularization.</ArticleTitle><Pagination><MedlinePgn>1-13</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jconrel.2014.05.010</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0168-3659(14)00313-7</ELocationID><Abstract><AbstractText>Recent cell-based therapy approaches have employed both nanotechnologies and other biomedical technologies to enhance their therapeutic potential. A combined strategy using therapeutic stem/progenitor cells and angiogenic proteins is attractive for the treatment of vascular disease. In this study, we developed an injectable multifunctional micro-sized gel system (microgel), composed of arginine-glycine-aspartic acid (RGD)-conjugated alginate, for the delivery of both cells and growth factors in vivo. The microgels encapsulated with outgrowth endothelial cells (OECs) and growth factors (vascular endothelial growth factor, VEGF, and hepatocyte growth factor, HGF) were formed via electrospraying. Cells encapsulated within the microgel exhibited a time-dependent proliferation with enhanced cell viability, and the size-controlled microgels resulted in sustained release of growth factors for enhanced new vessel formation by tube formation and rat aorta sprouting in vitro. Increased angiogenesis was also estimated in mice treated with RGD-microgel containing OECs and growth factors. Furthermore, injection of the multifunctional microgel into a hindlimb ischemia model improved blood flow perfusion and increased the capillary density by histological analysis. Compared with hydrogel system, injectable microgel system was shown to be superior with no toxicity. Overall, our injectable multifunctional microgel system can be attributed to deliver potential therapeutic agents/cells for the treatment of vascular diseases. </AbstractText><CopyrightInformation>Copyright © 2014. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Pyung-Hwan</ForeName><Initials>PH</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yim</LastName><ForeName>Hyun-Gu</ForeName><Initials>HG</Initials><AffiliationInfo><Affiliation>School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Young-Jin</ForeName><Initials>YJ</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Byung-Jae</ForeName><Initials>BJ</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Joohyun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kwon</LastName><ForeName>Sang-Mo</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Department of Physiology, School of Medicine, Pusan national University, Yangsan, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Byung-Soo</ForeName><Initials>BS</Initials><AffiliationInfo><Affiliation>School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hwang</LastName><ForeName>Nathaniel S</ForeName><Initials>NS</Initials><AffiliationInfo><Affiliation>School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Je-Yoel</ForeName><Initials>JY</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, South Korea. Electronic address: jeycho@snu.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>05</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Control Release</MedlineTA><NlmUniqueID>8607908</NlmUniqueID><ISSNLinking>0168-3659</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000464">Alginates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005782">Gels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006603">Hexuronic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009842">Oligopeptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D042461">Vascular Endothelial Growth Factor A</NameOfSubstance></Chemical><Chemical><RegistryNumber>27432CM55Q</RegistryNumber><NameOfSubstance UI="D012710">Serum Albumin, Bovine</NameOfSubstance></Chemical><Chemical><RegistryNumber>67256-21-7</RegistryNumber><NameOfSubstance UI="D017228">Hepatocyte Growth Factor</NameOfSubstance></Chemical><Chemical><RegistryNumber>78VO7F77PN</RegistryNumber><NameOfSubstance UI="C047981">arginyl-glycyl-aspartic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>8A5D83Q4RW</RegistryNumber><NameOfSubstance UI="D020723">Glucuronic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>I223NX31W9</RegistryNumber><NameOfSubstance UI="D016650">Fluorescein-5-isothiocyanate</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000464" MajorTopicYN="N">Alginates</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001011" MajorTopicYN="N">Aorta</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064987" MajorTopicYN="N">Cell- and Tissue-Based Therapy</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042783" MajorTopicYN="Y">Endothelial Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005121" MajorTopicYN="N">Extremities</DescriptorName><QualifierName UI="Q000098" MajorTopicYN="N">blood supply</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016650" MajorTopicYN="N">Fluorescein-5-isothiocyanate</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005782" MajorTopicYN="N">Gels</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020723" MajorTopicYN="N">Glucuronic Acid</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057809" MajorTopicYN="N">HEK293 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017228" MajorTopicYN="N">Hepatocyte Growth Factor</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006603" MajorTopicYN="N">Hexuronic Acids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D061307" MajorTopicYN="N">Human Umbilical Vein Endothelial Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007267" MajorTopicYN="N">Injections</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007511" MajorTopicYN="N">Ischemia</DescriptorName><QualifierName UI="Q000188" MajorTopicYN="N">drug therapy</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018919" MajorTopicYN="N">Neovascularization, Physiologic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009842" MajorTopicYN="N">Oligopeptides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017207" MajorTopicYN="N">Rats, Sprague-Dawley</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012039" MajorTopicYN="N">Regional Blood Flow</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012710" MajorTopicYN="N">Serum Albumin, Bovine</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042461" MajorTopicYN="N">Vascular Endothelial Growth Factor A</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cell therapy</Keyword><Keyword MajorTopicYN="N">Electrospraying</Keyword><Keyword MajorTopicYN="N">Neovascularization</Keyword><Keyword MajorTopicYN="N">Outgrowth endothelial cells</Keyword><Keyword MajorTopicYN="N">RGD-conjugated microgel</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>01</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>04</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>05</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>2</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24852096</ArticleId><ArticleId IdType="pii">S0168-3659(14)00313-7</ArticleId><ArticleId IdType="doi">10.1016/j.jconrel.2014.05.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Kim, Pyung Hwan" sort="Kim, Pyung Hwan" uniqKey="Kim P" first="Pyung-Hwan" last="Kim">Pyung-Hwan Kim</name></region><name sortKey="Cho, Je Yoel" sort="Cho, Je Yoel" uniqKey="Cho J" first="Je-Yoel" last="Cho">Je-Yoel Cho</name><name sortKey="Choi, Young Jin" sort="Choi, Young Jin" uniqKey="Choi Y" first="Young-Jin" last="Choi">Young-Jin Choi</name><name sortKey="Hwang, Nathaniel S" sort="Hwang, Nathaniel S" uniqKey="Hwang N" first="Nathaniel S" last="Hwang">Nathaniel S. Hwang</name><name sortKey="Kang, Byung Jae" sort="Kang, Byung Jae" uniqKey="Kang B" first="Byung-Jae" last="Kang">Byung-Jae Kang</name><name sortKey="Kim, Byung Soo" sort="Kim, Byung Soo" uniqKey="Kim B" first="Byung-Soo" last="Kim">Byung-Soo Kim</name><name sortKey="Kim, Joohyun" sort="Kim, Joohyun" uniqKey="Kim J" first="Joohyun" last="Kim">Joohyun Kim</name><name sortKey="Kwon, Sang Mo" sort="Kwon, Sang Mo" uniqKey="Kwon S" first="Sang-Mo" last="Kwon">Sang-Mo Kwon</name><name sortKey="Yim, Hyun Gu" sort="Yim, Hyun Gu" uniqKey="Yim H" first="Hyun-Gu" last="Yim">Hyun-Gu Yim</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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